Translational_Unit
AT-Bph

Part:BBa_K646001:Design

Designed by: David Dubow   Group: iGEM11_Macquarie_Australia   (2011-09-29)

Agrobacterium tumefaciens Bacteriophotochrome (Rhizobium radiobacter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1867
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal NgoMIV site found at 1694
    Illegal AgeI site found at 1759
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains two internal Pst1 restriction endonuclease sites that was removed via site directed mutagenesis from ctgcag to cttcag.

Source

From 2010 Macquarie Team, isolated from Genomic DNA

References